黑人无码一区二区

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    聯系方式

    E-mail:yanyi@pku.edu.cn
    辦公電話:+86-010-62744058
    辦公地點:北京大學綜合科研樓生物醫學前沿創新中心307室
    郵件地址:北京市海淀區頤和園路5號北京大學綜合科研樓307室,100871

    研究組主頁:http://gene.pku.edu.cn

    黃巖誼
    BIOPIC、ICG 研究員
    個人履歷

    2022.3-2024,北京大學生物醫學前沿創新中心(BIOPIC),副主任

    2020.3-現在,北京大學化學與分子工程學院,教授

    2016.8-現在,北京未來基因診斷高精尖創新中心(ICG),研究員

    2015-現在,北大-清華生命科學聯合中心,研究員

    2010-現在,北京大學生物醫學前沿創新中心(BIOPIC),研究員

    2013-2020,北京大學工學院,教授

    2006-2013,北京大學工學院,特聘研究員

    2005-2006,美國斯坦福大學生物工程系,博士后

    2002-2005,美國加州理工學院應用物理系,博士后

    2002,北京大學,博士

    1997,北京大學,理學學士

    主要研究方向

    黃巖誼課題組致力于發展應用于面向生命科學和醫學研究的新技術,課題組當前的研究興趣集中在微量樣品多組學技術、微流控技術及類器官培養技術三個方面。我們一直在發展高效、新型的高通量DNA測序技術及其應用方法,特別是測序化學與設備研發、單細胞測序技術應用與器件研發、以及少量細胞的表觀基因組學測序方法研發。我們同時大力發展大規模集成微流控芯片技術,并將其應用在生命科學關鍵問題的研究中,包括細胞培養及微環境控制,以及在單細胞層次上觀察生命過程的隨機性。我們還大力發展新的生物成像技術及圖像處理算法,用于研究細胞及活生物體的動態過程。我們利用相干拉曼散射顯微技術,可以無需標記來研究活生物體和活細胞內的三維化學成像,同時利用這樣的技術來促進藥物釋放及癌癥診斷的研究。

    獲獎及榮譽

    2015,國家自然基金委員會,杰出青年科學基金

    2014,  英國皇家化學會會士

    2013,北京大學綠葉生物醫學獎

    2012,國家自然科學基金委員會,優秀青年基金

    2010,第十二屆霍英東青年教師基金

    2009,北京大學青年教師教學演示競賽,一等獎

    2008,國家教育部新世紀優秀人才

    2004,全國優秀博士學位論文

    2003,國家自然科學二等獎,第四完成人

    2001,北京大學學生五四獎章

    2000,中國大學生五四獎學金

    代表性論文及論著
    1. Ruoyan Li,# Lin Di,# Jie Li,# Wenyi Fan,# Yachen Liu, Wenjia Guo, Weiling Liu, Lu Liu, Qiong Li, Liping Chen, Yamei Chen, Chuanwang Miao, Hongjin Liu, Yuqian Wang, Yuling Ma, Deshu Xu, Dongxin Lin*, Yanyi Huang*, Jianbin Wang*, Fan Bai*, Chen Wu*. A body map of somatic mutagenesis in morphologically normal human tissues. Nature 2021, 597 (7876), 398-403.
    2. Lin Di, Yusi Fu, Yue Sun, Jie Li, Lu Liu, Jiacheng Yao, Guanbo Wang, Yalei Wu, Kaiqin Lao, Raymond W Lee, Genhua Zheng, Jun Xu, Juntaek Oh, Dong Wang, X. Sunney Xie*, Yanyi Huang*, Jianbin Wang*. RNA Sequencing by Direct Tagmentation of RNA/DNA Hybrids. PNAS 2020, 117, 2886-2893.
    3. Xiannian Zhang, Tianqi Li, Feng Liu, Yaqi Chen, Jiacheng Yao, Zeyao Li, Yanyi Huang, Jianbin Wang. VVV1. Comparative analysis of droplet-based ultra-high-throughput single-cell RNA-seq systems. Molecular Cell. 2019 73(13) 130-142.e5
    4. Geng, S (Geng, Shuang); Huang, YY (Huang, Yanyi).  From Mouth Pipetting to Microfluidics: The Evolution of Technologies for Picking Healthy Single Cells.ADVANCED BIOSYSTEMS. 2018  2(9)UNSP 1800099.?
    5. Zhang, Y (Zhang, Yi); Gao, JX (Gao, Jiaxin); Huang, YY (Huang, Yanyi); Wang, JB (Wang, Jianbin). Recent Developments in Single-Cell RNA-Seq of Microorganisms.  BIOPHYSICAL JOURNAL,  2018 115(2)173-180.
    6. Jiang, Dongqing; Zhang, Xiannian; Pang, Yuhong; Zhang, Jianyun; Wang, Jianbin; Huang, Yanyi. Terminal transfer amplification and sequencing for high-efficiency and low-bias copy number profiling of fragmented DNA samples. Protein & cell.  2018.
    7. Chen, H (Chen, He); Yao, JC (Yao, Jiacheng); Fu, YS (Fu, Yusi); Pang, YH (Pang, Yuhong); Wang, JB (Wang, Jianbin); Huang, YY (Huang, Yanyi). Tagmentation on Microbeads: Restore Long-Range DNA Sequence Information Using Next Generation Sequencing with Library Prepared by Surface-Immobilized Transposomes. ACS APPLIED MATERIALS & INTERFACES.  2018  10(14)11539-11545.
    8. Chen Z, Liao P, Zhang F, et al. Centrifugal micro-channel array droplet generation for highly parallel digital PCR. Lab Chip 2017;17(2):235-40. doi: 10.1039/c6lc01305h
    9. Li S, Chen T, Wang Y, et al. Conjugated Polymer with Intrinsic Alkyne Units for Synergistically Enhanced Raman Imaging in Living Cells. Angew Chem Int Ed Engl 2017;56(43):13455-58. doi: 10.1002/anie.201707042
    10. Liao P, Huang Y. Digital PCR: Endless Frontier of ‘Divide and Conquer’. Micromachines 2017;8(8):231.
    11. Chen X, Huang Y. Editorial overview: Molecular imaging for seeing chemistry in biology. Curr Opin Chem Biol 2017;39:iv-v. doi: 10.1016/j.cbpa.2017.06.016
    12. Chen Z, Zhou W, Qiao S, et al. Highly accurate fluorogenic DNA sequencing with information theory-based error correction. Nat Biotechnol 2017;35(12):1170-78. doi: 10.1038/nbt.3982
    13. Chen T, Huang Y. Label-Free Transient Absorption Microscopy for Red Blood Cell Flow Velocity Measurement in Vivo. Anal Chem 2017;89(19):10120-23. doi: 10.1021/acs.analchem.7b01952
    14. Zheng C, Zhang X, Li C, et al. Microfluidic Device for Studying Controllable Hydrodynamic Flow Induced Cellular Responses. Anal Chem 2017;89(6):3710-15. doi: 10.1021/acs.analchem.7b00013
    15. Yang L, Ma Z, Cao C, et al. MR-seq: measuring a single cell’s transcriptome repeatedly by RNA-seq. Science Bulletin 2017;62(6):391-98. doi: https://doi.org/10.1016/j.scib.2017.01.029
    16. Wu AR, Wang J, Streets AM, et al. Single-Cell Transcriptional Analysis. Annu Rev Anal Chem (Palo Alto Calif) 2017;10(1):439-62. doi: 10.1146/annurev-anchem-061516-045228
    17. Li C, Yu Z, Fu Y, et al. Single-Cell-Based Platform for Copy Number Variation Profiling through Digital Counting of Amplified Genomic DNA Fragments. ACS Applied Materials & Interfaces 2017;9(16):13958-64. doi: 10.1021/acsami.7b03146
    18. Yujiao Dang,# Liying Yan,# Boqiang Hu,# Xiaoying Fan, Yixin Ren, Rong Li, Ying Lian, Jie Yan, Qingqing Li, Yan Zhang, Min Li, Xiulian Ren, Jin Huang, Yuqi Wu, Ping Liu, Lu Wen, Chen Zhang, Yanyi Huang,* Fuchou Tang,* Jie Qiao* .Tracing the expression of circular RNAs in human pre-implantation embryos. Genome Biology 17 130 (2016) .doi: 10.1186/s13059-016-0991-3 (2016) .doi: 10.1021/acs.analchem.6b00862
    19. Yusi Fu, Chunmei Li, Sijia Lu, Wenxiong Zhou, Fuchou Tang, X. Sunney Xie,* Yanyi Huang*. Uniform and accurate single-cell sequencing based on emulsion whole-genome amplification. Proceedings of the National Academy of Sciences of the United States of America 112 (38), 11923-11928 (2015) .doi: 10.1073/pnas.1513988112
    20. Xiaoying Fan,# Xiannian Zhang,# Xinglong Wu, Hongshan Guo, Yuqiong Hu, Fuchou Tang,* Yanyi Huang*. Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos. Genome Biology 16 148 (2015) .doi: 10.1186/s13059-015-0706-1
    21. Jie Shen,# Dongqing Jiang,# Yusi Fu,# Xinglong Wu,# Hongshan Guo, Binxiao Feng, Yuhong Pang, Aaron M. Streets, Fuchou Tang,* Yanyi Huang*. H3K4me3 epigenomic landscape derived from ChIP-Seq of 1,000 mouse early embryonic cells. Cell Research 25 (1), 143-147 (2015) .doi: 10.1038/cr.2014.119
    22. Aaron M. Streets, Xiannian Zhang, Chen Cao, Yuhong Pang, Xinglong Wu, Liang Xiong, Lu Yang, Yusi Fu, Liang Zhao,* Fuchou Tang,* Yanyi Huang.* Microfluidic single-cell whole transcriptome sequencing. PNAS 2014, published online. doi: 10.1073/pnas.1402030111
    23. Senlian Hong, Tao Chen, Yuntao Zhu, Ang Li, Yanyi Huang,* Xing Chen.* Live-cell stimulated Raman scattering imaging of alkyne-tagged biomolecules. Angew. Chem. Int. Ed. 2014, published online. doi: 10.1002/anie.201400328
    24. Aaron M. Streets, Yanyi Huang.* Chip in a lab: microfluidics for next generation life science research. Biomicrofluidics 2013, 7(1), 011302.
    25. Chunhong Zheng, Zhilong Yu, Ying Zhou, Louis Tao, Yuhong Pang, Tao Chen, Xiannian Zhang, Haiwei Qiu, Hongwei Zhou, Zitian Chen, Yanyi Huang.* Live cell imaging analysis of the epigenetic regulation of the human endothelial cell migration at single cell resolution. Lab on a Chip 2012, 12(17), 3063-3072.
    26. Zhilong Yu, Tao Chen, Xiannian Zhang, Dan Fu, Xin Liao, Jie Shen, Xinxing Liu, Bo Zhang, X. Sunney Xie, Xiaodong Su, Junyuan Chen,* Yanyi Huang.* Label-free chemical imaging in vivo: three-dimensional non-invasive microscopic observation of amphioxus notochord through stimulated Raman scattering (SRS). Chemical Science 2012, 3(8), 2646-2654.
    27. Chunhong Zheng, Jingwen Wang, Yuhong Pang, Jianbin Wang, Wenbin Li, Zigang Ge,* Yanyi Huang.* High-throughput immunoassay through in-channel microfluidic patterning. Lab on a Chip 2012, 12(14), 2487-2490. (featured as inside front cover)
    28. Yongfan Men, Yusi Fu, Zitian Chen, Peter A. Sims, William J. Greenleaf, Yanyi Huang.* Digital polymerase chain reaction in an array of femtoliter PDMS microreactors. Analytical Chemistry 2012, 84(10), 4262-4266.
    29. Wentao Li, Tao Chen, Zitian Chen, Peng Fei, Zhilong Yu, Yuhong Pang, Yanyi Huang.* Squeeze-chip: A finger controlled microfluidic flow network device and its application to biochemical assays. Lab on a Chip 2012, 12(9), 1587-1590. (featured as inside front cover)
    30. Peng Fei, Zhilong Yu, Xu Wang, Peter J. Lu, Yusi Fu, Zi He, Jingwei Xiong, Yanyi Huang.* High dynamic range optical projection tomography (HDR-OPT). Optics Express 2012, 20(8), 8824-8836.
    31. Ying Zhou, Yuhong Pang, Yanyi Huang.* Openly Accessible Microfluidic Liquid Handlers for Automated High-throughput Nanoliter Cell Culture. Analytical Chemistry 2012, 84(5), 2576-2584.
    32. Chunhong Zheng, Liang Zhao, Gui&rsquo;e Chen, Ying Zhou, Yuhong Pang, Yanyi Huang.* Quantitative Study of the Dynamic Tumor-endothelial Cell Interactions through an Integrated Microfluidic Coculture System. Analytical Chemistry 2012, 84(4), 2088-2093.
    33. Jie Shen, Ying Zhou, Tu Lu, Junya Peng, Zhixiang Lin, Lei Huang, Yuhong Pang, Li Yu, Yanyi Huang.* An Integrated Chip for Immunofluorescence and Its Application to Analyze Lysosomal Storage Disorders. Lab on a Chip 2012, 12, 317-324.
    34. Zuoyan Han, Wentao Li, Yanyi Huang, Bo Zheng. Measuring rapid enzymatic kinetics by electrochemical method in droplet-based microfluidic devices with pneumatic valves. Analytical Chemistry 2009, 81(14), 5840-5845.
    35. Jianbin Wang, Ying Zhou, Haiwei Qiu, Huang Huang, Changhong Sun, Jianzhong Xi, Yanyi Huang.* A Chip-to-chip nanoliter microfluidic dispenser. Lab on a Chip 2009, 9(13), 1831-1835.
    36. Yuanzi Wu, Yanyi Huang, Hongwei Ma. A facial method for permanent and functional surface modification of poly(dimethylsiloxane). Journal of the American Chemical Society 2007, 129(23), 7226-7227.
    37. Yanyi Huang, Piero Castrataro, Cheng-Chung Lee, Stephen R. Quake. Solvent resistant microfluidic DNA synthesizer. Lab on a Chip 2007, 7(1), 24-26.
    38. Wei Liang, Lan Yang, Joyce K. S. Poon, Yanyi Huang,* Amnon Yariv, Kerry Vahala. Transmission characteristics of a Fabry-Perot etalon-microtoroid resonator coupled system. Optics Letters 2006, 31(4), 510-512.
    39. Wei Liang, Yanyi Huang,* Yong Xu, Reginald K. Lee, Amnon Yariv. Highly-sensitive fiber Bragg grating refractive index sensors. Applied Physics Letters 2005, 86(15), 151122.
    40. Yanyi Huang, Yong Xu, Amnon Yariv. Fabrication of functional microstructured optical fibers through a selective filling technique. Applied Physics Letters 2004, 85(22), 5182-5184.
    41. Yanyi Huang,* George T. Paloczi, Joyce K. S. Poon, Amnon Yariv. Bottom-up softlithographic fabrication of three-dimensional multilayer polymer integrated optical microdevices. Applied Physics Letters 2004, 85(15), 3005-3007.
    42. Yanyi Huang,* George T. Paloczi, Amnon Yariv, Cheng Zhang, Larry R. Dalton. Fabrication and Replication of Polymer Integrated Optical Devices Using Electron-Beam Lithography and Soft Lithography. Journal of Physical Chemistry B 2004, 108(25), 8606-8613.
    43. Yanyi Huang,* George T. Paloczi, Joyce K. S. Poon, Amnon Yariv. Demonstration of flexible freestanding all-polymer integrated optical ring resonator devices. Advanced Materials 2004, 16(1), 44-48.