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    首頁» 中心PI» 白凡
    聯系方式

    E-mail:fbai@pku.edu.cn
    辦公電話:010-62756164
    辦公地點:綜合科研2號樓303室

    研究組主頁:

    白凡
    BIOPIC副主任、研究員
    個人履歷

    2024-至今 北京大學生物醫學前沿創新中心(BIOPIC),副主任

    2024.03-至今 北大-清華生命科學聯合中心研究員

    2022-至今 北京大學生物醫學前沿創新中心(BIOPIC),教授

    2020-至今 北京未來基因診斷高精尖創新中心(ICG),研究員

    2014-至今 北京大學生物醫學前沿創新中心(BIOPIC),研究員

    2011-2014 北京大學生物醫學前沿創新中心(BIOPIC),副研究員

    2009-2011 日本大阪大學先端生命機能研究科,JSPS博士后

    2008-2009 英國牛津大學物理系,博士后

    2008 英國牛津大學,理學博士

    2003 北京大學,理學學士

    主要研究方向

    2011年全職回國之后,白凡實驗室致力于創新和應用單細胞技術,探索生物醫學前沿交叉領域:1)在癌癥病人外周血循環腫瘤細胞(CTCs)研究上取得重大突破,在世界范圍第一個報道單個CTC的全基因組測序結果(PNAS, 2013; Genome Research, 2017),推進CTCs作為腫瘤無創診斷標志物的臨床應用(Clinical Cancer Research, 2019),獲得廣泛關注,作為團隊重要成員獲2019年國家科技進步二等獎;2)發展激光顯微切割結合微量細胞測序技術,實現石蠟病理切片下特殊形態區域的精確分離,低起始量的外顯子測序。深入研究了肝癌的各種肝內轉移病灶的異質性(Gastroenterology, 2016),揭示肝癌轉移復雜的分子機制與混合型肝癌形成原因(Cancer Cell, 2019, 細胞出版社2019中國年度論文)。深入研究了多發性尿路上皮癌的致癌機制和多病灶間的克隆演化關系(European Urology, 2017),首次發現形態正常尿路上皮組織存在大量突變和克隆擴增(Science, 2020),發現人體正常組織體細胞突變的組織器官分布規律和特異性(Nature, 2021);3)應用單細胞轉錄組測序及免疫分型技術,發現鼻咽癌腫瘤細胞的“上皮-免疫”雙重特性(Cell Research, 2020),揭示了兒童炎癥性腸病的關鍵致病機制和治療靶標(Cell, 2019, 中國臨床醫學十大進展),揭示了兒童膽道閉鎖的關鍵致病機制和治療方法(Cell, 2020);4)結合單細菌熒光成像和測序技術,深入探究了細菌耐藥性產生的分子機制(Molecular Cell 2016; Molecular Cell 2019;獲得Nature重點評述)。實驗室研究方向:研究方向一:單細胞基因測序技術應用于癌癥病人外周血循環腫瘤細胞研究,深入探索癌癥轉移的分子生物學機制及通過循環腫瘤細胞測序進行無創癌癥診斷、預后判斷、療效評價的臨床方法。研究方向二:大樣本基因測序研究癌癥發生、轉移機制。針對明確的臨床問題,采用基因測序手段研究中國人群高發癌癥的基因突變特征,發掘引起癌癥表型轉化、轉移的關鍵驅動事件。研究方向三:綜合使用基因測序、熒光成像手段研究細菌行為、致病性,抗生素殺菌原理和細菌抗藥、耐藥的分子機制。

    獲獎及榮譽

    ?2022,勃林格殷格翰資深研究員獎

    ?2021,中源協和生命醫學創新突破獎

    ?2021,樹蘭醫學獎-青年獎

    2021,國家杰出青年科學基金

    2017,國家優秀青年科學基金

    2014,北京大學綠葉生物醫藥杰出青年學者獎

    2011,青年海外高層次人才計劃

    2007,英國牛津大學WellcomeTrust VIP 研究學者

    2004,中國-牛津學者

    代表性論文及論著

    ?(*indicates corresponding or co-corresponding authors, # indicates equal contributed first authors)

    單細胞測序腫瘤基因組學方向

    Single-cell Sequencing and Cancer Genomics

    1. Li R#, Di L#, Li J#, Fan W#, etc., Lin D*, Huang Y*, Wang J*, Bai F*, Wu C*, A body map of somatic mutagenesis in morphologically normal human tissues, Nature 597 (2021), 398-403
    2. Wang J#, Xu Y#, Chen Z#, Liang J#, Lin Z#, Liang H#, ……, Bai F*, Xia H*, Wen Z*, Zhang Y*, Liver immune profiling reveals pathogenesis and therapeutics for biliary atresia, Cell 183 (2020), pp. 1867-1883
    3. Li R#, Du Y#, Chen Z#, Xu D#, Lin T#, Jin S, Wang G, Liu Z, Lu M, Chen X, Xu T*, Bai F*, Macroscopic somatic clonal expansion in morphologically normal human urothelium, Science 370 (2020), pp. 82-89
    4. Jin S#, Li R#, Chen M#, Yu C#, Tang L#, …….., Mai H, Gewurz B, Zhao B, Young L, Zhong Q*, Bai F*, Zeng M*, Single-cell transcriptomic analysis defines the interplay between tumor cells, viral infection, and the microenvironment in nasopharyngeal carcinoma, Cell Research 30 (2020), pp. 950-965
    5. Zhang J#, Wang X#, Xing X#, Xu Z#, ……., Zhang Y, Zumla A, Maeurer M, Bai F*, Wang F*, Single-cell landscape of immunological responses in patients with COVID-19, Nature Immunology 21 (2020), pp. 1107-1118
    6. Zhang C#, Zhang L#, Xu T#, Xue R#, Yu L#, Zhu Y, Wu Y, Zhang Q, Li D, Shen S, Tan D, Bai F*, Zhang H*, Mapping the spreading routes of lymphatic metastases in human colorectal cancer, Nature Communications 11 (2020), 1993
    7. Wang X#, Lu Y#, Song Y#, Dong J#, Li R, Wang G, Wang X, Zhang S, Dong Z, Lu M, Wang S, Ge L, Luo G, Ma R, Rozen S, Bai F*, Wu D*, Ma L*, Integrative genomic study of Chinese clear cell renal cell carcinoma reveals features associated with thrombus, Nature Communications 11 (2020), 739
    8. Huang B#, Chen Z#, Geng L#, Wang J#, Liang H#, ......, Gong S*, Bai F*, Yang M*, Zhang Y*, Mucosal profiling of pediatric-onset colitis and IBD reveals common pathogenics and therapeutic pathways, Cell 179 (2019), pp. 1160-1176
    9. Fujimaki K#, Li R#, Chen H, Della Croce K, Zhang H, Xing J, Bai F*, Yao G*, Graded regulation of cellular quiescence depth between proliferation and senescence by a lysosomal dimmer switch, Proceedings of the National Academy of Sciences USA 116 (2019), pp. 22624-22634
    10. Su Z#, Wang Z#, Ni X#, Duan J#, Gao Y#, Zhuo M, Li R, Zhao J, Ma Q, Bai H, Chen H, Wang S, Chen X, An T, Wang Y, Tian Y, Yu J, Wang D, Xie X*, Bai F*, Wang J*, Inferring evolution and progression of small-cell lung cancer by single-cell sequencing of circulating tumor cells, Clinical Cancer Research 25 (2019), pp. 5049-5060
    11. Xue R#, Chen L#, Zhang C#, Fujita M#, ……, Nakagawa H*, Zeng M*, Bai F*, Zhang N*, Genomic and transcriptomic profiling of combined hepatocellular and intrahepatic cholangiocarcinoma reveals distinct molecular subtypes, Cancer Cell 35 (2019), pp. 932-947
    12. Jiang Y#, Ma D#, Suo C#, Shi J#, Xue M#, Liu Y, Xiao Y, Hu X, Yu Y, Zhang J, Li B, Li X, Zheng Y, Ren L, Hou W, Zhao S, Gong Y, Ren Y, Zhang C, Niu Z, Bai F, Yu K, Wang P*, Shi L*, Huang W*, Shao Z*, The genomic and transcriptomic landscape of triple-negative breast cancer suggests subtype-specific treatment strategies in Chinese population, Cancer Cell 35 (2019), pp.428-440.e5
    13. Liu M#, Liu Y#, Deng L#, Wang D, He X, Zhou L, Wicha M, Bai F*, Liu S*, Transcriptional profiles of different states of cancer stem cells in triple-negative breast cancer, Molecular Cancer 17(2018), pp. 65
    14. Chen X#, Yan S#, Liu Y#, Zhong Q#, Chen Z#, Jin S#, Xia T, Li R, Zhou A, Su Z, Huang Y, Huang Q, Huang L, Zhang X, Zhao Y, Yun J, Wu Q, Lin D, Bai F*, Zeng M*, Genomic comparison of esophageal squamous cell carcinoma and its precursor lesions by multi-region whole exome sequencing, Nature Communications 8 (2017), pp. 524
    15. Gao Y#, Ni X#, Guo H#, Su Z#, Ba Y#, Tong Z, Guo Z, Yao X, Chen X, Yin J, Yan Z, Guo L, Liu Y, Bai F*, Xie S*, Zhang N*, Single-cell sequencing deciphers a convergent evolution of copy number alterations from primary to circulating tumor cells, Genome Research 27 (2017), pp. 1312-1322
    16. Du Y#, Li R#, Chen Z#, Wang X, Xu T*, Bai F*, Mutagenic factors and complex clonal relationship of multifocal urothelial cell carcinoma, European Urology 71 (2017), pp. 841–843
    17. Xue R#, Li R#, Guo H#, Guo L#, Su Z, Ni X, Qi L, Zhang T, Li Q, Zhang Z, Xie XS, Bai F*, Zhang N*, Variable extent of intra-tumor heterogeneity revealed by genomic sequencing of multiple lesions in patients with hepatocellular carcinoma, Gastroenterology 150 (2016), pp. 998-1008
    18. Ni X#, Zhuo M#, Su Z#, Duan J#, Gao Y#, Wang Z#, Zong C#, Bai H, Chapman A, Zhao J, Xu L, An T, Ma Q, Wang Y, Wu M, Sun Y, Wang S, Li Z, Yang X, Yong J, Su X, Lu Y, Bai F*, Xie X*, Wang J*, Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients, Proceedings of the National Academy of Sciences USA 110 (2013), pp. 21083-21088
    19. Lu S#, Zong C#, Fan W#, Yang M#, Li J, Chapman A, Zhu P, Hu X, Xu L, Yan L, Bai F, Qiao J, Tang F, Li R*, Xie X*, Probing meiotic recombination and aneuploidy of single sperm cells by whole genome sequencing, Science 338 (2012), pp. 1627-1630

    細菌生理及抗生素耐藥

    Bacterial Physiology and Antibiotic Tolerance

    1. Zhuang X#, Guo S#, Li Z#, Zhao Z#, Kojima S, Homma M, Wang P, Lo C*, Bai F*, Live-cell fluorescence imaging reveals dynamic production and loss of bacterial flagella, Molecular Microbiology (2020), (April 07, 2020 online)
    2. Wang Y#, Tian T#, Zhang J#, Jin X#, Yue H, Zhang X, Du L, Bai F*, Indole reverses the intrinsic antibiotic resistance of Lysobacter by promoting the expression of a novel dual-function importer, mBio 10 (2019), e00676-19
    3. Pu Y#, Li Y#, Jin X#, Tian T#, Ma Q, Zhao Z, Lin S, Chen Z, Li B, Leake MC, Lo CJ, Bai F*, ATP-dependent dynamic protein aggregation regulates bacterial dormancy depth critical for antibiotic tolerance, Molecular Cell 73 (2019), pp. 1-14 (November 21, 2018 online)
    4. Zhao Z#, Zhao Y#, Zhuang X#, Lo W, Baker MA, Lo C*, Bai F*, Frequent pauses in Escherichia coli flagella elongation revealed by single cell real-time fluorescence imaging, Nature Communications 9 (2018), pp. 1885
    5. Chen M#, Zhao Z#, Yang J#, Peng K, Baker M, Bai F*, Lo C*, Length-dependent flagellar growth of Vibrio alginolyticus revealed by real time fluorescent imaging, eLife (2017), DOI: http://dx.doi.org/10.7554/eLife.22140
    6. Pu Y, Ke Y*, Bai F*, Active efflux in dormant bacterial cells – new insights into antibiotic persistence, Drug Resistance Updates 30 (2017), pp. 7-14 (invited review)
    7. Buda R#, Liu Y#, Yang J#, Hegde S#, Stevenson K, Bai F*, Pilizota T*, Dynamics of Escherichia coli’s passive response to a sudden decrease in external osmolarity, Proceedings of the National Academy of Sciences USA 113 (2016), pp. E5838-E5846
    8. Pu Y#, Zhao Z#, Li Y#, Zou J, Ma Q, Zhao Y, Ke Y, Zhu Y, Chen H, Baker A.B. M, Ge H, Sun Y, Xie XS*, Bai F*, Enhanced efflux activity facilitates drug tolerance in dormant bacterial cells, Molecular Cell 62 (2016), pp. 284-294
    9. Ma Q, Sowa Y, Baker M*, Bai F*, Conformational spread in the flagellar motor switch in response to CheY-P regulation and motor structural alterations, Biophysical Journal 110 (2016), pp. 1411-1420
    10. Xue R#, Ma Q#, Baker M, Bai F*, A delicate nanoscale motor made by nature-the bacterial flagellar motor, Advanced Science 2 (2015), pp. 1500129 (invited review)
    11. Bai F*, Che YS, Kami-ike N, Ma Q, Minamino T, Sowa Y, Namba K*, Populational heterogeneity vs. temporal fluctuation in Escherichia coli flagellar motor switching, Biophysical Journal 105 (2013), pp. 2123-2129
    12. Bai F, Minamino T, Wu Z, Namba K*, Xing J*, Coupling between switching regulation and torque generation in bacterial flagellar motor, Physical Review Letters 108 (2012), pp. 178105
    13. Ma Q, Nicolau D, Berry R, Maini P. Bai F*, Conformational spread in the flagellar motor switch: a model study, PLoS Computational Biology 8 (2012), pp. e1002523
    14. Bai F#, Branch R#, Nicolau D#, Pilizota T, Steel, B, Maini P, Berry R*, Conformational spread as a mechanism for cooperativity in the bacterial flagellar switch, Science 327 (2010), pp. 685-689
    15. Bai F, Lo C, Berry R, Xing J*, Model studies on the dynamics of bacterial flagellar motor, Biophysical Journal 96 (2009), pp. 3154-3167
    16. Leake MC, Chandler JH, Wadhams GH, Bai F, Berry RM, Armitage JP*, Stoichiometry and turnover in single, functioning membrane protein complexes, Nature 443 (2006), pp. 355-358 
    17. Xing J, Bai F, Berry R, Oster G*, Torque-speed relationship of the bacterial flagellar motor, Proceedings of the National Academy of Sciences USA 103 (2006), pp. 1260-1265